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FIGHT-TWO

About the Project

The strategic framework FIGHT-TWO (PTDC / CVT-CVT / 29062/2017-PT2020) is the development and production of an oral and safe vaccine against RHDV2 for the wild rabbit.

This edible vaccine will be distributed in the field as bait or dry food and has the potential to protect a large proportion of the wild rabbits populations, crucial for reducing virus transmission and controlling infection, overcoming the need for capture and handling of the animals.

SUMMARY OF ACTIVITIES

ACTIVITY 1

Construction of recombinant plasmids containing the RHDV2-vp60 gene.

ACTIVITY 2

Production and purification of RHDV2 VP60-VLPs; Structural and functional evaluation of VP60-VLPs.

ACTIVITY 3

Production of an immune serum against RHDV2 in a domestic rabbit using a commercial vaccine; Implementation of the test of Inhibition of Haemagllutination HI (according to the OIE manual) and development of an indirect ELISA (I-ELISA) for the specific detection of RHDV2 antibodies developed in orally inoculated rabbits.

ACTIVITY 4

Evaluation of the resistance of the VP60-VLPs to temperature, humidity and lyophilisation.

ACTIVITY 5

Testing the oral route for RHDV2-vaccine immunization of domestic and wild rabbits; Challenge with infectious virus to evaluate immunological protection conferred by the vaccine.

ACTIVITY 6

Testing the VP60-VPL-vaccine by oral route by immunization of wild rabbits kept in herd facility; Challenge with RHDV2.

ACTIVITY 7

Evaluation of the duration of the immunological protection and Statistical Analysis.

ACTIVITY 8

Results dissemination and Patent application.

COLLABORATIVE PLATFORM

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